Predictive value of the sulfidoleukotriene release assay in oral allergy syndrome to celery, hazelnut, and carrot.

نویسندگان

  • B K Ballmer-Weber
  • J M Weber
  • S Vieths
  • B Wüthrich
چکیده

BACKGROUND Patients sensitized to birch pollen frequently suffer from a food allergy to plant foods such as celery, carrots, or hazelnut. One of the main manifestations of birch pollen-related food allergy is the oral allergy syndrome. Skin tests and allergen-specific immunoglobulin (Ig) E determinations are poor predictors of such reactions when assessed by double-blind placebo-controlled food challenge (DBPCFC). OBJECTIVE To investigate whether a cellular test based on leukotriene release from basophils, the cellular antigen stimulation test in combination with enzyme-linked immunosorbent assay (CAST-ELISA), is predictive of pollen-related food allergy. METHODS Birch pollen-sensitized patients with positive DBPCFC to celery (n=21), hazelnut (n=15), and carrot (n=7) underwent skin tests along with determination of specific IgE and CAST-ELISA for the respective allergens. The results were compared with those of 24 birch pollen-sensitized patients with negative open food challenge to celery, hazelnut, and carrot. RESULTS While skin prick tests had a sensitivity of 85%, 80%, and 29% for commercial extracts of celery, hazelnut, and carrot, respectively, prick testing with self-prepared extracts yielded sensitivities of 100%, 80%, and 100%, respectively. For specific IgE determinations, sensitivities were 71%, 73%, and 57%, respectively, and the respective specificities were 67%, 73%, and 60%. For CAST-ELISA with various sources and doses of allergens, the sensitivity varied from 71% to 95% for celery, 73% to 80% for hazelnut, and 43% to 86% for carrot. The respective specificities were 67% to 92%, 75% to 88%, and 77% to 91%. Analysis of the predictive value of CAST-ELISA with receiver operating characteristic curves showed that the results of the tests were more predictive of pollen-related food allergy than quantitative allergen-specific IgE determinations. CONCLUSIONS CAST-ELISA is more specific than routine diagnostic tests for the diagnosis of pollen-related food allergy to celery, hazelnut, and carrot.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Diagnosis of cross-reactivity in patients with birth pollinosis

Material and Methods The clinical history and the positive SPT with the birch extract were the condition for qualifications. 23 patients included in the first group were only birch allergic. 35 patients in the other group had birch pollen allergy and they reported symptoms after eating apple, celery, carrot, tomato, banana, peach, peanut and hazelnut. The skin tests were performed and serum IgE...

متن کامل

Anaphylactic shock induced by Codonopsis lanceolata, traditional Chinese medicine in a patient with allergic rhinitis.

Codonopsis lanceolata (CL) contains various active components including tannins, saponins, polyphenolics, alkaloids, essential oils and steroids. Therefore, it has been prescribed as a traditional folk medicine to treat several inflammatory diseases (1, 2). To date, there have been no published reports of allergic reactions to CL. In this report, we present a serious anaphylactoid reaction foll...

متن کامل

Lipid transfer protein cross-reactivity assessed in vivo and in vitro in the office: pros and cons.

BACKGROUND Few studies analyze cross-reactivity between lipid transfer proteins (LTP) from a large spectrum of botanically unrelated plant-derived foods using routine diagnostic tests. OBJECTIVE To assess the clinical usefulness of currently available in vivo and in vitro tests in LTP-hypersensitive patients. METHODS An in vitro and in vivo study was performed of 15 peach-allergic adults mo...

متن کامل

Effect of celery,sour apple and carrot in some of serum biochemical parameters of diabetic rats

In order to investigate the effect of celery,sour apple and carrot on some of the biochemical parameters of serum from diabetic rats,25 male adult rats were selected.the rats were divided into 5 groups.a group consisting of 5 rats were chosen as the control group and diabetes mellitus was induced in 20 out of 25 adult male albino rats using intraperitoneal injection of 170mg/kg(body weight) of ...

متن کامل

Food allergy population thresholds: DBPCFC data for rice, kiwi, apple, peach, carrot, maize, tomato, and other less common allergenic foods

The “Big 8” food allergens consists of cow’s milk, eggs, wheat, peanuts, tree nuts, soybeans, fish, and crustacean shellfish and account for ~90% of the allergic reactions to foods. During the scientific review of the VITAL (Voluntary Incidental Trace Allergen Labelling) program, individual challenge data were gathered for the Big 8 food allergens (plus mustard, sesame and celery) and, where po...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of investigational allergology & clinical immunology

دوره 18 2  شماره 

صفحات  -

تاریخ انتشار 2008